期刊
BIOLOGY OF REPRODUCTION
卷 79, 期 5, 页码 962-975出版社
SOC STUDY REPRODUCTION
DOI: 10.1095/biolreprod.107.067546
关键词
apoptosis; germ cell differentiation; germ cell proliferation; meiosis; spermatogenesis in vitro
资金
- Governmental and European Community funds
- Foundation for Science and Technology
- Ministry of Science, Technology, and Superior Education [SFRH/BD/23616/2005, POCI/SAU-MMO/60709/60555/59997/04
- UMIB]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/23616/2005] Funding Source: FCT
In vitro culturing of normal human seminiferous epithelium remains largely unexplored. To study normal human spermatogenesis in vitro, we used a micromethod for the purification and culture of Sertoli cells, spermatogonia A, spermatocytes, and early round spermatids. Cytological quantitative data for Sertoli and premeiotic germ cell cocultures isolated from normal testicular biopsies demonstrated that cells were able to proliferate (4%), complete meiosis (6.7%), and differentiate into late round (54%), elongating (49%), and elongated (17%) spermatids at similar in vivo time delays (up to 16 days) in response to FSH + testosterone stimulation. Cells maintained normal meiotic segregation, chromosome complements, and specific gene expression profiles. Follicle-stimulating hormone + testosterone stimulated spermatogonia proliferation and Sertoli cell survival. Follicle-stimulating hormone and especially FSH + testosterone increased diploid germ cell survival during the first week, whereas only FSH + testosterone was able to inhibit cell death during the second week of culture. Follicle-stimulating hormone and especially FSH + testosterone also stimulated meiosis resumption, although this was restricted to late pachytene and secondary spermatocytes. In contrast, spermiogenesis was only stimulated by FSH + testosterone. Expression studies showed that apoptosis was induced in the nucleus of diploid cells, and in nuclear and cytoplasmic compartments of spermatids, mainly triggered by the Fas pathway. Although junctional complexes between Sertoli and premeiotic germ cells were partially reacquired, the same did not apply to spermatids, suggesting that FSH potentiated by testosterone was unable to render Sertoli cells competent to bind round spermatids.
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