4.4 Article

Expression, purification, crystallization and preliminary X-ray analysis of the i-carrageenase from Alteromonas fortis

期刊

出版社

INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/S0907444900004844

关键词

-

向作者/读者索取更多资源

This is the first crystallization report of a glycoside hydrolase which belongs to family 82. A recombinant form of His-tagged iota-carrageenase from Alteromonas fortis was expressed, purified and crystallized. Crystals were obtained by the vapour-diffusion method using polyethylene glycol (M-W = 6000) as a precipitant. They belong to space group P2(1), with unit-cell parameters a = 56.75, b = 91.04, c = 125.01 Angstrom, beta = 93.41 degrees. The unit cell contains two molecules in the asymmetric unit related by a non-crystallographic twofold axis. Crystals diffracted to 2.0 Angstrom resolution on a synchrotron beamline.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.4
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据