期刊
ANALYTICA CHIMICA ACTA
卷 878, 期 -, 页码 1-8出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2015.04.036
关键词
Upconversion nanoparticles; Quantum dots; Luminescence resonance energy transfer; Nucleic acid hybridization; Bioassay; Paper
资金
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- Ontario Ministry of Training, College and Universities (MTCU)
- NSERC
Monodisperse aqueous upconverting nanoparticles (UCNPs) were covalently immobilized on aldehyde modified cellulose paper via reduction amination to develop a luminescence resonance energy transfer (LRET)-based nucleic acid hybridization assay. This first account of covalent immobilization of UCNPs on paper for a bioassay reports an optically responsive method that is sensitive, reproducible and robust. The immobilized UCNPs were decorated with oligonucleotide probes to capture HPRT1 housekeeping gene fragments, which in turn brought reporter conjugated quantum dots (QDs) in close proximity to the UCNPs for LRET. This sandwich assay could detect unlabeled oligonucleotide target, and had a limit of detection of 13 fmol and a dynamic range spanning nearly 3 orders of magnitude. The use of QDs, which are excellent LRET acceptors, demonstrated improved sensitivity, limit of detection, dynamic range and selectivity compared to similar assays that have used molecular fluorophores as acceptors. The selectivity of the assay was attributed to the decoration of the QDs with polyethylene glycol to eliminate nonspecific adsorption. The kinetics of hybridization were determined to be diffusion limited and full signal development occurred within 3 min. (C) 2015 Elsevier B.V. All rights reserved.
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