期刊
FEMS MICROBIOLOGY LETTERS
卷 187, 期 1, 页码 41-45出版社
ELSEVIER SCIENCE BV
DOI: 10.1111/j.1574-6968.2000.tb09134.x
关键词
positive selection; vector; transformation; Cryptococcus neoformans
类别
We report the engineering of a new shuttle vector featuring its episomal maintenance in Cryptococcus neoformans and the lethal Escherichia coli ccdB gene for positive selection in bacteria. Telomere-like sequences from C. neoformans and the STAB fragment confer episomal maintenance to the vector (pPM8) upon transformation in C. neoformans. The vector generated high transformation frequencies and each transformant was estimated to harbor thirty copies of the plasmid. The plasmids recovered in E. coli from the C. neoformans transformants showed no evidence of rearrangement. This construct will be very useful for cloning and studying the regulation of genes in C. neoformans. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
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