期刊
TRANSPLANT IMMUNOLOGY
卷 8, 期 2, 页码 125-128出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0966-3274(00)00019-8
关键词
flow cytometric crossmatch; complement-dependent cytotoxicity crossmatch; antibody; low-level immunization; kidney transplantation
Flow-PRA(R) is a flow cytometric method for both anti-HLA class I and class II antibody (Ab) detection. We evaluated this technique for Ab screening in patients awaiting kidney transplantation. After having established a rigorous threshold for positivity, a three-dilution difference in sensitivity between Flow-PRA(R) and complement-dependent cytotoxicity (CDC) persisted. The sensitivity of the method was satisfactory since all CDC-positive sera were also found to be positive with the Flow-PRA(R) method. Discrimination between anti-HLA class I and class II Abs was excellent. Furthermore, all sera responsible for a positive flow cytometry crossmatch (FCXM) and a negative CDC-crossmatch (CDC,XM) at the time of a putative transplant were found to be positive with Flow-PRA(R) beads. The specificity was excellent for anti-class I Ab detection since no false positive serum was found. On the other hand, the specificity was lower for anti-class II detection, since 8.3% (2/24) false positive results were detected among all the negative sera tested. Overall, our results suggested that Flow-PRA(R) should be of value for anti-HLA Ab screening prior to kidney transplantation. (C) 2000 Elsevier Science B.V. All rights reserved.
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