期刊
JOURNAL OF PHYSIOLOGY-LONDON
卷 525, 期 2, 页码 331-341出版社
CAMBRIDGE UNIV PRESS
DOI: 10.1111/j.1469-7793.2000.00331.x
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资金
- NINDS NIH HHS [NS38715, R01 NS038715] Funding Source: Medline
1. Whole-cell patch-clamp recordings were made from subthalamic nucleus (STN) neurones in brain slices from rats. Stimulation with bipolar electrodes evoked synaptic currents mediated by glutamate (EPSCs) and GABA(A) (IPSCs) receptors. 2. Dopamine reversibly reduced the amplitude of GABA(A), IPSCs by up to 48% with an IC50 value of 3.4 +/- 0.8 mu M. The dopamine D-2 receptor agonist quinpirole, but not the D-1 receptor agonist SHF 82958, also inhibited GABA(A) IPSCs. This effect was completely reversed by the D-2 receptor antagonist sulpiride but not by SCH 23390, a D-1 antagonist. 3. Muscarine reversibly reduced the amplitude of GABA(A) IPSCs by up to 70% with an IC50 value of 0.6 +/- 0.1 mu M. Inhibition of IPSCs by muscarine was completely blocked by scopolamine (10 mu M), a muscarinic receptor antagonist. The M-3 muscarinic receptor antagonist 4-DAMP effectively reversed muscarine-induced inhibition of IPSCs with an IC50 of 0.11 +/- 0.03 mu M. Although the M-1 receptor antagonist pirenzepine also reversed the inhibition of IPSCs by muscarine, this effect was only observed at relatively high concentrations (IC50 = 21.7 +/- 9.4 mu M). 4. Dopamine and muscarine both increased the paired-pulse ratio of GABA(A) IPSCs. Neither agent produced sustained changes in postsynaptic holding current. 5. Glutamate EPSCs were also inhibited reversibly by dopamine (by up to 29%; IC50 = 16 +/- 3 mu M) and muscarine (by up to 41%; IC50 = 1.0 +/- 0.4 mu M). However, both agents were more potent and efficacious for reducing GABA IPSCs compared with glutamate EPSCs. 6. These results suggest that the most significant effect of dopamine and muscarine in the STN is to reduce inhibitory synaptic input by acting at presynaptic dopamine D-2 and muscarinic M-3 receptors, respectively.
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