期刊
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
卷 278, 期 6, 页码 L1248-L1255出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajplung.2000.278.6.L1248
关键词
ClC-2; chloride channels; rat
资金
- NHLBI NIH HHS [KO8-HL-03469] Funding Source: Medline
The fetal lung actively transports chloride across the airway epithelium. ClC-2, a pH-activated chloride channel, is highly expressed in the fetal lung and is located on the apical surface of the developing respiratory epithelium. Our goal was to determine whether acidic pH could stimulate chloride secretion in fetal rat distal lung epithelial cells mounted in Ussing chambers. A series of acidic solutions stimulated equivalent short-circuit current (I-eq) from a baseline of 28 +/- 4.8 (pH 7.4) to 70 +/- 5 (pH 6.2), 114 +/- 12.8 (pH 5.0), and 164 +/- 19.2 (pH 3.8) mu A/cm(2). These changes in I-eq were inhibited by 1 mM cadmium chloride and did not result in large changes in [H-3]mannitol paracellular flux. Immunofluorescent detection by confocal microscopy revealed that ClC-2 is expressed along the luminal surface of polarized fetal distal lung epithelial cells. These data suggest that the acidic environment of the fetal lung fluid could activate chloride channels contributing to fetal lung fluid production and that the changes in I-eq seen in these Ussing studies may be due to stimulation of ClC-2.
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