期刊
BIOSENSORS & BIOELECTRONICS
卷 15, 期 3-4, 页码 203-209出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/S0956-5663(00)00061-0
关键词
porous gold; ellipsometry; protein adsorption; adsorption models; biosensor
The sensitivity of optical biosensors where the detection takes place on a planar gold surface can be improved by making the surface porous. The porosity allows a larger number of ligands per surface area resulting in larger optical shifts when interacting with specifically binding analyte molecules. The porous gold was deposited as a thin layer on a planar gold surface by electrochemical deposition in a solution of tetrachloroaurate and lead acetate. A protein, streptavidin, was adsorbed into the formed porous layer and the time course of the adsorption was monitored by in-situ ellipsometry. When the porous layer was 500 nm in thickness a six-fold increase of the ellipsometric response was obtained compared with a planar gold surface. The dependency of porosity and layer thickness was explained with a mathematical model of the gold/porous gold/protein/solution system. (C) 2000 Elsevier Science S.A. All rights reserved.
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