期刊
BIOCHEMICAL JOURNAL
卷 348, 期 -, 页码 417-423出版社
PORTLAND PRESS
DOI: 10.1042/0264-6021:3480417
关键词
glycolipids; phosphocholine; on-target enzymic cleavage; parasitic filarial nematodes
Human Onchocerca volvulus infection sera were found to recognize zwitterionic glycolipids of O. volvulus and to cross-react with those of other parasitic nematodes (Ascaris suum, Setaria digitata and Litomosoides sigmodontis). By the use of an epitope-specific monoclonal antibody, zwitterionic glycolipids of all these nematode species were observed to contain the antigenic determinant phosphocholine. A hyperimmune serum specific for arthro-series glycolipid structures reacted with the various neutral glycolipids of all these nematodes, which demonstrated that their oligosaccharide moieties belonged to the arthro-series of protostomial glycolipids. These results indicated that arthro-series glycosphingolipids carrying, in part, phosphocholine substituents, represent highly conserved, antigenic glycolipid markers of parasitic nematodes. Three glycolipid components of the O. volvulus zwitterionic fraction were structurally characterized by matrix-assisted laser-desorption/ionization time-of-flight MS, methylation analysis and exoglycosidase treatment. Their chemical structures were elucidated to be phosphocholine-6GlcNAc(beta 1-3)Man(beta 1-4)Glc(1-1)ceramide, GalNAc(beta 1-4)-[phosphocholine-6] GlcNAc(beta 1-3)Man(beta 1-4)Glc(l-l)ceramide and Gal(alpha 1-3)GalNAc(beta 1-4)[phosphocholine-6]GlcNAc(beta 1-3)-Man(beta 1-4)Glc(1-1)ceramide for the zwitterionic ceramide tri-, tetra- and penta-hexosides respectively. The ceramide composition was found to be dominated by 2-hydroxylated docosanoic (C-22h:0), tricosanoic (C-23h:0) and tetracosanoic (C-24h:0) acids, and C-17 sphingosine (C-d17:1) (where (h) is hydroxylated and (d) is dihydroxylated).
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