期刊
BRITISH JOURNAL OF CANCER
卷 82, 期 11, 页码 1808-1813出版社
CHURCHILL LIVINGSTONE
DOI: 10.1054/bjoc.2000.1111
关键词
cell adhesion molecules; carbonic anhydrase; tumour immunology; phage display; drug design
类别
MN/CA IX is a cell surface protein, strongly associated with several types of human carcinomas. It exerts activity of carbonic anhydrase and capacity of binding to cell surface receptors. in the present work, we used affinity purified MN/CA IX protein to demonstrate that the cells adhere to immobilized MN/CA IX and that the monoclonal antibody M75 abrogates cell attachment to MN/CA IX, Using synthetic oligopeptides, we identified M75 epitope and located it in the proteoglycan domain, which contains a sixfold tandem repeat of six amino acids GEEDLP. From phage display library of random heptapeptides we identified and chemically synthesized those which compete for the epitope with M75 and inhibit adhesion of cells to MN/CA IX. These heptapeptides might serve as lead compounds for drug design. (C) 2000 Cancer Research Campaign.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据