Determination of moxifloxacin (BAY 12-8039) in plasma and lung tissue by high-performance liquid chromatography with ultraviolet detection using a fully automated extraction method with a new polymeric cartridge

The aim of this study was to develop a. high-performance liquid chromatographic (HPLC) assay for the determination of moxifloxacin in human plasma and lung tissue. The assay was based on HPLC with a Supelcosil ABZ+ column and ultraviolet detection set at a wavelength of 296 nm, The extraction procedure was characterized by a fully automated liquid-solid extraction using an OASIS column for the solid phase. The assay has been found to be linear and validated over the concentration range 3.2 to 0.025 mu g/ml for moxifloxacin in plasma and from 16 to 0.25 mu g/g for moxifloxacin in lung tissue. Tn future, the assay will support the pharmacokinetic study of the penetration of moxifloxacin in human lung tissue, (C) 2000 Elsevier Science B.V. All rights reserved.