期刊
JOURNAL OF CHROMATOGRAPHY B
卷 742, 期 2, 页码 353-362出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0378-4347(00)00180-8
关键词
glucagon; insulin
资金
- NIDDK NIH HHS [DK46960] Funding Source: Medline
A rapid capillary electrophoresis (CE) with laser-induced fluorescence (LIF) competitive immunoassay has been developed for the determination of glucagon in biological mixtures. In the assay, fluorescein-conjugated glucagon is mixed with the sample followed by addition of anti-glucagon. Free and antibody-bound, tagged glucagon could be separated in 3 s using CE to obtain quantitative determination of glucagon with a concentration detection limit of 760 pM. The assay was combined with a previously developed competitive immunoassay for insulin to produce a simultaneous immunoassay for both peptides. The method was used to determine glucagon content of islets of Langerhans. (C) 2000 Elsevier Science B.V. All rights reserved.
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