Enhanced calcium transients in glial cells in neonatal cerebellar cultures derived from S100B null mice

S100B is the major low-affinity Ca2+-binding protein in astrocytes. In order to study the role of S100B in the maintenance of Ca2+ homeostasis, we generated S100B null mice by a targeted inactivation of the S100B gene. Absence of S100B expression was demonstrated by Northern and Western blotting for S100B mRNA and protein, respectively, and immunoperoxidase staining of sections of various brain regions. S100B null mice were viable, fertile, and exhibited no overt behavioral abnormalities up to 12 months of age. On the basis of light microscopy and immunohistochemical staining, there were no discernable alterations in the distribution and morphology of astrocytes or neurons in sections of adult brains of these mice. Astrocytes in cerebellar cultures derived from 6-day-old S100B null mice exhibited enhanced Ca2+ transients in response to treatment with KCl or caffeine. On the other hand, granule neurons, in the same cultures, exhibited normal Ca2+ transients in response to treatment with KCl, caffeine, or N-methyl-D-aspartate. These results demonstrate a specific decrease in Ca2+-handling capacity in astrocytes derived from S100B null mice and suggest that S100B plays a role in the maintenance of Ca2+ homeostasis in astrocytes. (C) 2000 Academic Press.