期刊
NEUROSCIENCE LETTERS
卷 287, 期 1, 页码 65-67出版社
ELSEVIER SCI IRELAND LTD
DOI: 10.1016/S0304-3940(00)01153-8
关键词
alpha-synuclein; Parkinson's disease; cerebrospinal fluid; neurodegenerative diseases; biological markers
Several clues suggest that alpha-synuclein, a presynaptic protein, plays a central role in the pathogenesis of idiopathic Parkinson's disease (PD). To search a peripheral marker of PD, we analyzed presence and amount of alpha-synuclein in CSF from 12 PD patients and 10 neurologically normal subjects. The protein was extracted from CSF samples through immunoprecipitation and immunoblotting with different specific anti-alpha-synuclein antibodies. We identified a 19 kDa band that corresponds to monomeric alpha-synuclein, given its comigration with homologue human recombinant peptide as well as with the protein extracted from cerebral cortex of normal subjects. The amount of CSF 19 kDa alpha-synuclein did not significantly vary in PD and normal cases. These findings have two implications: (a) full length alpha-synuclein is released by neurons in the extracellular space; (b) alpha-synuclein does not appear a peripheral marker of PD pathology. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.
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