期刊
JOURNAL OF CHROMATOGRAPHY A
卷 883, 期 1-2, 页码 103-112出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0021-9673(00)00378-2
关键词
preparative chromatography; microcystins; toxins; peptides
Preparative reversed-phase liquid chromatography was successfully used to purify two microcystins (microcystin LR and microcystin LA) from a cyanobacterial process waste. The separation protocol involved extraction of lyophilized cells by methanol, isolation and concentration by solid-phase extraction, and purification by reversed-phase HPLC. Milligram-level loading of microcystins was obtained on a solid-phase extraction cartridge packed with 0.5 g of C-18 stationary phase. The separations were first carried out on an analytical column and then scaled-up to a preparative column. The microcystins were quantified by HPLC and enzyme-linked immunosorbent assay. A method to remove microcystins rapidly and economically from the cyanobacterial process waste is also described. (C) 2000 Elsevier Science B.V. All rights reserved.
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