期刊
JOURNAL OF CHROMATOGRAPHY B
卷 743, 期 1-2, 页码 349-356出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0378-4347(00)00223-1
关键词
aqueous two-phase systems; protein partitioning; enzyme purification; Bacillus pumilus; alkaline xylanase
The aim of this work was to extract and to purify xylanase, produced by Bacillus pumilus from the crude fermentation broth, using aqueous two-phase systems (ATPS). The xylanase was extracted by partitioning in ATPS composed of phosphate and polyethylene glycol (PEG). The effect of tie-line length, PEG molecular mass and NaCl concentrations upon the purification factors and yields of xylanase were investigated by statistical design. The best system studied was that containing 22% PEG6000, 10% K2HPO4 and 12% NaCl with a purification factor of 33 and a 98% yield of enzyme activity. This system was also used for continuous extraction in a pulsed caps column. Subsequently, the xylanase from the crude fermentation broth was tested in hardwood kraft pulp bleaching. (C) 2000 Elsevier Science B.V. All rights reserved.
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