期刊
TALANTA
卷 52, 期 3, 页码 509-515出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0039-9140(00)00394-5
关键词
cysteine; 2-chloro-1-methylpyridinium iodide; derivatization; HPLC; plasma
This paper describes the development and validation of a reversed-phase HPLC method with isocratic conditions for the analysis of total cysteine in human plasma. The essential steps in the assay include derivatization of cysteine via thiol group with 2-chloro-1-methylpyridinium iodide (CMPI), separation of the formed cysteine S-pyridinium derivative from endogenous thiol derivatives, usually present in biological samples, by ion-pair reversed-phase HPLC, and ultraviolet detection. Free cystine and protein-bound cysteine are converted to their reduced counterpart by the use of 2-mercaptoethanol. The standard curve is linear over the concentration range from 20 to 300 nmol ml(-1). The recoveries are from 93.9 to 105.90% and imprecision was at most 2.5%. Optimal conditions are determined for all steps of the analytical procedure. (C) 2000 Elsevier Science B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据