期刊
JOURNAL OF VIROLOGY
卷 74, 期 13, 页码 6198-6202出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.74.13.6198-6202.2000
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资金
- NCI NIH HHS [T32 CA009141, T32 CA09141] Funding Source: Medline
- NIAID NIH HHS [AI28691, R37 AI029873, AI29873, P30 AI028691, R01 AI029873] Funding Source: Medline
Mature human immunodeficiency virus type 1 (HIV-1) virions contain a typically cone-shaped core that encases the viral genome. In this study, we established conditions which allowed the efficient isolation of morphologically intact HIV-1 cores from virions. The isolated cores consisted mostly of cones which appeared uniformly capped at both ends but were heterogeneous with respect to the shape of the broad cap as well as the dimensions and angle of the cone. Vpr, a nonstructural virion component implicated in the nuclear import of the viral genome, was recovered in core preparations of HIV-1 and simian immunodeficiency viruses from African green monkeys, Unexpectedly, p6(gag), a structural protein required for the incorporation of Vpr, was absent from HIV-1 core preparations. Taken together, our results indicate that the incorporation of Vpr into the virion core is a conserved feature of primate lentiviruses and that the interactions required for the uptake of Vpr into assembling particles differ from those which confine Vpr within the core.
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