期刊
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY
卷 279, 期 1, 页码 R222-R229出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpregu.2000.279.1.R222
关键词
teleost fish; mRNA expression; salinity acclimation; developmental maturation; immunolocalization
类别
Recent studies on teleost fish have suggested that their genomes have undergone ancient polyploidization events resulting in the duplication of the genome. A duplicate copy of the Na,K-ATPase beta(1)-isoform (called beta(233)) has been identified in the European eel (Anguilla anguilla). The beta(233)-isoform shares high levels of nucleotide (74.8%) and amino acid (69.9%) homology with the eel beta(1)-subunit as well as other vertebrate beta(1)-sequences. Compared with the widely expressed beta(1)-isoform, expression of beta(233)-mRNA is mainly restricted to epithelial tissues. Seawater acclimation induced increases in beta(233)-mRNA levels in kidney, gill, and intestine of migratory silver but not the nonmigratory yellow adult eels, suggesting that the factors responsible for this upregulation are themselves developmentally regulated. Expression of a variably glycosylated 40- to 52-kDa beta(233)-protein in both gill chloride and intestinal epithelial cells suggests that the beta(233)-isoform of Na,K-ATPase may play an important functional role in the major osmoregulatory tissues of euryhaline fish such as the eel.
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