期刊
COORDINATION CHEMISTRY REVIEWS
卷 204, 期 -, 页码 1-112出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/S0010-8545(99)00218-0
关键词
ESEEM; HYSCORE; metalloproteins; C-13; N-15; O-17
The applications of electron spin echo envelope modulation (ESEEM) spectroscopy to study paramagnetic metal centers in metalloproteins and bioinorganic complexes are reviewed, with special attention to the novel spectroscopic techniques applied and the structural information obtained. We summarize the physical principles and experimental techniques of ESEEM, the spectral shapes and the methods for their analysis. The physical meaning of the spin Hamiltonian parameters is highlighted in conjunction with their utilization for obtaining structural information. (C) 2000 Elsevier Science S.A. All rights reserved.
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