期刊
JOURNAL OF APPLIED MICROBIOLOGY
卷 89, 期 1, 页码 16-23出版社
BLACKWELL SCIENCE LTD
DOI: 10.1046/j.1365-2672.2000.01087.x
关键词
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A soil isolate designated 90-F-45-14, belonging to Bacillus thuringiensis serovar dakota (H15), was examined for characterization of in vitro cytotoxicity, associated with parasporal inclusion proteins, against human cells. When activated with proteolytic processing, inclusion proteins of the isolate 90-F-45-14 exhibited a moderate cytotoxicity against the human uterus cervix cancer cells (HeLa) with an EC50 value of 60.8 mu g ml(-1) while showing extremely high activities on the human leukaemic T cells (MOLT-4) and the normal T cells with EC50 values of 0.27 and 0.20 mu g ml(-1), respectively. Anti-leukaemic cell activity of the 90-F-45-14 proteins was eight to nine times greater than that of the B. thuringiensis serovar israelensis proteins containing the Cyt1 protein, a broad-spectrum cytolysin. The cytopathy by the 90-F-45-14 proteins was characterized by marked cell-ballooning, while the israelensis proteins induced early breakdown of the cells due to cytolysis. Inclusions of the isolate consisted of five major polypeptides of 170, 103, 73, 40 and 32 kDa. A 100% homology was observed in the sequence of 15 N-terminal amino acids between the proteins of 170 and 103 kDa. There was no N-terminal sequence homology between 90-F-45-14 proteins and the existing Cry/Cyt proteins of B. thuringiensis, Proteolytic processing by proteinase K yielded several proteins with molecular masses ranging from 40 to 28 kDa.
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