期刊
ARCHIVES OF MICROBIOLOGY
卷 174, 期 1-2, 页码 125-128出版社
SPRINGER-VERLAG
DOI: 10.1007/s002030000185
关键词
Saccharomyces cerevisiae; Zygosaccharomyces bailii; fluorescence-ratio-imaging microscopy; short-term pH(i) response; acetic acid; individual cells
类别
The effects of perfusion with 2.7 and 26 mM undissociated acetic acid in the absence or presence of glucose on short-term intracellular pH (pH(i)) changes in individual Saccharomyces cerevisiae and Zygosaccharomyces bailii cells were studied using fluorescence-ratio-imaging microscopy and a perfusion system. In the S. cerevisiae cells, perfusion with acetic acid induced strong short-term pH, responses, which were dependent on the undissociated acetic acid concentration and the presence of glucose in the perfusion solutions. In the Z. bailii cells, perfusion with acetic acid induced only very weak shortterm pHi responses, which were neither dependent on the undissociated acetic acid concentration nor on the presence of glucose in the perfusion solutions. These results clearly show that Z. bailii is more resistant than S. cerevisiae to short-term pHi changes caused by acetic acid.
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