期刊
MYCOLOGICAL RESEARCH
卷 104, 期 -, 页码 858-864出版社
ELSEVIER SCI LTD
DOI: 10.1017/S0953756299002075
关键词
-
类别
The fatty acid composition of pea roots with and without the root pathogen Aphanomyces euteiches was studied using whole cell fatty acids (WCFA), phospholipid fatty acids (PLFA) and neutral lipid fatty acids (NLFA). Initial screening for A. euteiches markers using WCFA analysis showed that the fatty acids 14:1 omega 9, 14:0, 20:4 and 20:5 were specific to roots infected with A. enteiches. The amounts of these fatty acids correlated positively with the percentage of the root system containing A. euteiches oospores, suggesting that they could be useful indicators of A. euteiches mot-infection levels. Analysis of the composition of phospholipid fatty acids (PLFA) and neutral lipid fatty acids (NLFA) of pea roots with and without A. euteiches showed similar specificity as found with WCFA analysis. PLFAs and NLFAs specific to roots inoculated with A. euteiches were also present in mycelium from pure culture of A. euteiches. The PLFAs 14:0, 20:4 and 20:5 were used to estimate biomass of A. euteiches and the NLFAs 14:1 omega 9, 14:0, 20:4 and 20:5 were used as indicators of energy reserves of the pathogen. The possible use of specific fatty acids to study growth and development of A. euteiches in pea roots is discussed.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据