Expression of porcine adipocyte transcripts during differentiation in vitro and in vivo

Transcript concentrations for the transcription factors, CCAAT enhancer binding protein beta and alpha (C/EBP beta and C/EBP alpha), plus the adipocyte-characteristic proteins, fatty acid synthase (FAS), glucose transporter 4 (Glut. 3), hormone-sensitive lipase (HSL), insulin receptor (InsR), lipoprotein lipase (LPL), and leptin were measured during differentiation of porcine stromal-vascular (S/V) cells in vitro. These same transcripts, excluding FAS and InsR, were measured in por cine adipose tissue from birth to 7 weeks of age. In S/V cells, C/EBP beta and InsR were continuously elevated. At day 0, C/EBP alpha was approximate to 20% of the day 9 value. The LPL increased gradually from day 0 to 9, whereas most other transcripts had a lag period of several days. In tissue, C/EBP beta was substantial at birth and increased gradually. The C/EBP alpha was relatively low at birth and increased at day 17. The LPL and leptin increased continuously. The Glut 4 was low at birth and increased at day 28. The HSL was relatively low at birth, increased at day 10, and plateaued at day 28. Transcripts in porcine S/V cells develop somewhat differently from adipocyte differentiation models established in clonal cells, but the porcine cells represent a model that should be more applicable to pigs. (C) 2000 Elsevier Science Inc. All rights reserved.