期刊
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
卷 126, 期 3, 页码 291-302出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0305-0491(00)00185-1
关键词
CCAAT enhancer binding protein alpha C/EBP alpha; CCAAT enhancer binding protein beta C/EBP beta; fatty acid synthase; glucose transporter 4; hormone-sensitive lipase; insulin receptor; leptin; lipoprotein lipase
Transcript concentrations for the transcription factors, CCAAT enhancer binding protein beta and alpha (C/EBP beta and C/EBP alpha), plus the adipocyte-characteristic proteins, fatty acid synthase (FAS), glucose transporter 4 (Glut. 3), hormone-sensitive lipase (HSL), insulin receptor (InsR), lipoprotein lipase (LPL), and leptin were measured during differentiation of porcine stromal-vascular (S/V) cells in vitro. These same transcripts, excluding FAS and InsR, were measured in por cine adipose tissue from birth to 7 weeks of age. In S/V cells, C/EBP beta and InsR were continuously elevated. At day 0, C/EBP alpha was approximate to 20% of the day 9 value. The LPL increased gradually from day 0 to 9, whereas most other transcripts had a lag period of several days. In tissue, C/EBP beta was substantial at birth and increased gradually. The C/EBP alpha was relatively low at birth and increased at day 17. The LPL and leptin increased continuously. The Glut 4 was low at birth and increased at day 28. The HSL was relatively low at birth, increased at day 10, and plateaued at day 28. Transcripts in porcine S/V cells develop somewhat differently from adipocyte differentiation models established in clonal cells, but the porcine cells represent a model that should be more applicable to pigs. (C) 2000 Elsevier Science Inc. All rights reserved.
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