期刊
BIOLOGICAL TRACE ELEMENT RESEARCH
卷 157, 期 2, 页码 175-182出版社
HUMANA PRESS INC
DOI: 10.1007/s12011-013-9869-3
关键词
Ru(II)-Se complex; Telomeric G-quadruplex; Anticancer activity; Apoptosis; Cell migration; Antiangiogenesis
资金
- National Natural Science Foundation of China [20871056, 21171070]
- Planned Item of Science and Technology of Guangdong Province [c1011220800060]
- Natural Science Foundation of Guangdong Province
- Fundamental Research Funds for the Central Universities
A new Ru(II)-Se complex, Ru(bpy)(2)L2Cl2 (bpy = 2,2'-bipyridine, L = 1,10-phenanthrolineselenazole) (Ru-Se) has been synthesized and characterized. The G-quadruplex DNA-binding properties of the complex and its selenium ligand (Phen-Se) were evaluated by thermal denaturation study, polymerase chain reaction (PCR) stop assay, and telomerase repeat amplification protocol (TRAP). The results showed that the obtained complex could induce and stabilize G-quadruplex structure as well as exhibit potent inhibitory activity against telomerase. In vitro cytotoxicity studies showed that complex Ru-Se inhibited the cancer cell growth through apoptosis. However, the presence of the ligand Phen-Se did not appear to have a significant effect either on G-quadruplex binding or on biological activity. Furthermore, the cell migration assay and the tube formation assay also demonstrated that the complex Ru-Se significantly inhibited human umbilical vascular endothelial cell (HUVEC) proliferation, migration, and tube formation. These findings indicate that the Ru-Se complex may be a potential telomerase inhibitor and a viable drug candidate in antiangiogenesis for anticancer therapies.
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