期刊
EXPERIMENTAL PARASITOLOGY
卷 95, 期 3, 页码 187-195出版社
ACADEMIC PRESS INC
DOI: 10.1006/expr.2000.4535
关键词
Plasmodium yoelii; malaria; rhoptry protein; erythrocyte invasion
类别
资金
- Medical Research Council [MC_U117532067] Funding Source: Medline
- Medical Research Council [MC_U117532067] Funding Source: researchfish
- MRC [MC_U117532067] Funding Source: UKRI
The 235-kDa rhoptry protein of the rodent malaria parasite Plasmodium yoelii yoelii was shown to bind to the surface of mouse red blood cells in a calcium-independent process, using a erythrocyte-binding assay. This binding is affected by modification of the surface of the red blood cells by enzymatic treatment. Chymotrypsin and trypsin but not neuraminidase treatment of the erythrocytes significantly reduced the binding of the 235-kDa proteins. The binding of an unrelated 135-kDa protein was abolished by treatment with chymotrypsin. Although the 235-kDa proteins bind to both reticulocytes and mature red blood cells, the binding to mature cells was more pronounced. In the presence of hyperimmune infection serum or specific polyclonal antibodies to the 235-kDa protein its binding to erythrocytes was reduced, further demonstrating the specificity of this ligand-receptor interaction. (C) 2000 Academic Press.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据