期刊
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
卷 30, 期 7, 页码 579-589出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0965-1748(00)00031-X
关键词
heat shock protein; ecdysteroid; transcription; prothoracicotropic hormone; juvenile hormone
资金
- NIDDK NIH HHS [DK-30118] Funding Source: Medline
The brain neuropeptide prothoracicotropic hormone (PTTH) stimulates a rapid increase in ecdysteroid hormone synthesis that is accompanied by general and specific increases in protein synthesis, including that of a 70 kDa cognate heat shock protein (hse 70). To further understand the possible roles of hsc 70, hsc 70 cDNA clones were isolated from a tobacco hornworm (Manduca sexta) prothoracic gland cDNA library. All sequenced clones were highly homologous to the Drosophila hsc 70-4 isoform. Manduca hsc 70 mRNA levels during the last larval instar exhibited a peak at the onset of wandering and a peak that coincided with the major pre-metamorphic peak of ecdysteroid synthesis. Manipulations of the glands' hormonal milieu showed that hsc 70 mRNA levels respond to 20-hydroxyecdysone, dibutyryl cAMP, PTTH and the JH analogue hydroprene. The protein and mRNA data suggest that hsc 70 could be involved in a negative feedback loop regulating assembly of the ecdysone receptor complex. (C) 2000 Elsevier Science Ltd. All rights reserved.
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