Constituents of various wood-rotting basidiomycetes

Phytochemical investigation of n-hexane and methanol extracts of fruiting bodies of the wood-rotting fungi Fomitopsis pinicola, Ganoderma lipsiense. Fomes fomentarius and Gloeophyllum odoratum led to the isolation and identification of several triterpene derivatives and some aromatic compounds derived from lignin. These are the new natural products, namely, pinicolic acid E (16 alpha-hydroxy-3-oxolanosta-8,24-dien-21-oic acid) and pinicolol C (3-oxolanosta-7,9(11),24-trien-15 alpha,21 -diol) from the crust of F. pinicola, ganoderenic acid D {(E)-7 beta-hydroxy-3,11,15,23-tetraoxolanosta-8,20(22)-dien-26-oic acid} and ganoderic acid N (7 beta,20-dihydroxy-3,11,15,23-tetraoxolanost-8-en-26-oic acid) from G. lipsiense and ergosterol peroxide (5 alpha,8 alpha-epidioxyergost-6-en-3 beta-ol) as well as ergost-7-en-3-one from F. fomentarius. From G. odoratum, dehydroeburicoic acid {24-methylene-3-oxolanosta-7,9(11)-dien-21-oic acid}, the dimethylacetal of 4,4,14 alpha-trimethyl-24-oxo-5 alpha-chol-8-en-21-oic acid and some aromatic compounds, of which 1-(4'-methoxyphenyl)-1,2-ethandiol is a new natural product, were isolated. Furthermore, a complete set of C-13 NMR data of the steryl esters 3 beta-linoleyloxyergosta-7,24(28)-diene, 3 beta-linoleyloxyergosta-7,22-diene and 3 beta-linoleyloxyergost-7-ene, which could bt identified as a mixture in all investigated fungi, could be recorded. It was proved by HPLC and TLC investigations, that the crust on top of the fruiting bodies of F. pinicola consists of lanostane derivatives. (C) 2000 Elsevier Science Ltd. All rights reserved.