期刊
ARCHIVES OF ORAL BIOLOGY
卷 45, 期 7, 页码 563-568出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0003-9969(00)00023-6
关键词
hyaluronan; versican; link protein; dental pulp
资金
- NICHD NIH HHS [N01-HD-&-3263] Funding Source: Medline
Versican is a large interstitial proteoglycan that is believed to be able to bind hyaluronan to form large aggregate structures, but no study has isolated native versican aggregates from any tissue. In this study, ternary aggregate structures consisting of versican, hyaluronan, and link protein were isolated from rat dental pulp by associative extractions followed by caesium sulphate rate zonal sedimentation centrifugation. Fractions from the centrifugation were analysed by dot blot and Western blot using monoclonal antibodies and hyaluronan-binding protein. Abc ut 60% of the hexuronic acid was extracted by associative extractions. Positive reactions for versican, hyaluronan and link protein were clearly detected in the bottom fractions from the centrifugation, but were barely detectable in the top fractions. These results suggest that the majority of the versican, hyaluronan, and link protein forms ternary aggregate structures in the rat dental pulp. (C) 2000 Elsevier Science Ltd. All rights reserved.
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