期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 275, 期 27, 页码 20268-20273出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M000894200
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The presence of ATP within cells is well established However, ATP also operates as an intercellular signal cia specific purinoceptors, Furthermore, nonsecretory cells can release ATP under certain experimental conditions. To measure ATP release and membrane currents from a single cell simultaneously, we used Xenopus oocytes, We simultaneously recorded membrane currents and luminescence, Here, we show that ATP release can be triggered in Xenopus oocytes by hyperpolarizing pulses. ATP release (3.2 +/- 0.3 pmol/oocyte) generated a slow inward current (2.3 +/- 0.1 mu A). During hyperpolarizing pulses, the permeability for ATP(4-) was more than 4000 times higher than that for Cl-. The sensitivity to GdCl3 (0.2 mM) of hyperpolarization-induced ionic current, ATP release and E-ATPase activity suggests their dependence on stretch-activated ion channels. The pharmacological profile of the current inhibition coincides with the inhibition of ecto-ATPase activity. This enzyme is highly conserved among species, and in humans, it has been cloned and characterized as CD39, The translation, in Xenopus oocytes, of human CD39 mRNA encoding enhances the ATP-supported current, indicating that CD39 is directly or indirectly responsible for the electrodiffusion of ATP.
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