期刊
FEBS LETTERS
卷 476, 期 3, 页码 224-228出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(00)01753-1
关键词
sugar transport; ion channel reconstitution; current fluctuation; noise analysis
Sugar permeation through maltoporin of Escherichia coli, a trimer protein that facilitates maltodextrin translocation across outer bacterial membranes, was investigated at the single channel level. For large sugars, such as maltohexaose, elementary events of individual sugar molecule penetration into the channel a ere readily observed. At small sugar concentrations an elementary event consists of maltoporin channel closure by one third of its initial conductance in sugar-free solution. Statistical anal? sis of such closures at higher sugar concentrations shows that all three pores of the maltoporin channel transport sugars independently. interestingly, while channel conductance is only slightly asymmetric showing about 10% higher values at -200 mV than at +200 mV (from the side of protein addition), asymmetry in dependence of the sugar binding constant on the voltage polarity is about 20 times higher. Combining our data with observations made with bacteriophage-h we conclude that the sugar residence time is much more sensitive to (and is decreased by) voltages that are negative from the intra-cell side of the bacterial membrane. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
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