期刊
JOURNAL OF BIOTECHNOLOGY
卷 80, 期 3, 页码 241-247出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0168-1656(00)00265-0
关键词
hemicellulase; hydrolysis; lignin; black liquor; degradation; circular dichroism
The influence of lignin, lignin model compounds, and black liquor from the kraft pulping process on the hydrolysis of xylan by xylanase was investigated. Addition of vanillic acid, acetovanillone, and protocatechuic acid increased the rate of hydrolysis of xylan by as much as 18-50% at low concentrations, but reached maxims at about 0.05% concentration. Addition of vanillin caused a 15% improvement in xylan hydrolysis, while addition of guaiacol more than doubled the hydrolysis rate. Increasing concentrations of either lignin or black liquor also increased the hydrolysis rate of xylan. Circular dichroism spectroscopy indicated a change in the structure of xylanase in the presence of black liquor. (C) Published by Elsevier Science B.V.
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