期刊
JOURNAL OF BIOTECHNOLOGY
卷 80, 期 3, 页码 195-202出版社
ELSEVIER
DOI: 10.1016/S0168-1656(00)00248-0
关键词
Bacillus coagulans; BK07; ferulic acid; biodegradation; 4-vinylguaiacol; vanillin
A new strain Bacillus coagulans BK07 was isolated from decomposed wood-bark, based on its ability to grow on ferulic acid as a sole carbon source. This strain rapidly decarboxylated ferulic acid to 4-vinylguaiacol, which was immediately converted to vanillin and then oxidized to vanillic acid. Vanillic acid was further demethylated to protocatechuic acid. Above 95% substrate degradation was obtained within 7 h of growth on ferulic acid medium, which is the shortest period of time reported to date. The major degradation products, was isolated and identified by thin-layer chromatography, high performance liquid chromatography and H-1-nuclear magnetic resonance spectroscopy were 4-vinylguaiacol, vanillin, vanillic acid and protocatechuic acid. (C) 2000 Elsevier Science B.V. All rights reserved.
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