期刊
EMBO JOURNAL
卷 19, 期 14, 页码 3618-3629出版社
WILEY
DOI: 10.1093/emboj/19.14.3618
关键词
calcineurin; Cryptococcus neoformans; Down's syndrome; pathogenic fungi; protein phosphatase
资金
- NIAID NIH HHS [R37 AI039115, 5T32AI07392, T32 AI007392, P01 AI044975, R01AI39115, R01 AI039115, R01 AI042159, R01AI42159] Funding Source: Medline
Calcineurin is the conserved target of the immunosuppressants cyclosporin A and FK506, Using the yeast two-hybrid system, we identified a novel calcineurin binding protein, CBP1, from the pathogenic fungus Cryptococcus neoformans, We show that CBP1 binds to calcineurin in vitro and in vivo, and FKBP12-FK506 inhibits CBP1 binding to calcineurin, Cryptococcus neoformans cbp1 mutant strains exhibit modest defects in growth under stress conditions and virulence, similar to but less severe than the phenotypes of calcineurin mutants, Saccharomyces cerevisiae mutants lacking the CBP1 homolog RCN1 are, like calcineurin mutants, sensitive to lithium cation stress, CBP1 shares a central peptide sequence motif, SPPxSPP, with related proteins in S.cerevisiae, Schizosaccharomyces pombe, Drosophila melanogaster, Caenorhabditis elegans and humans, and peptides containing this motif altered calcineurin activity in vitro, Interestingly, the human CBP1 homolog DSCR1 is encoded by the Down's syndrome candidate region interval on chromosome 21, is highly expressed in the heart and central nervous system, and may play a role in calcineurin functions in heart development, neurite extension and memory.
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