期刊
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
卷 744, 期 2, 页码 273-282出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0378-4347(00)00258-9
关键词
metallothionein isoforms; ion-exchange HPLC; liver; rat
A sensitive method for determination of metallothionein (MT) isoform levels in rat liver by ion-exchange highperformance liquid chromatography and atomic absorption spectrometry was developed. Critical steps in sample preparation, like MT extraction. MT saturation with Cd and protein separation, were optimized. This method is capable of measuring levels of 2.0 mu g/g liver for metallothionein-1 (MT-1) and 1.3 mu g/g liver for metallothionein-2 (MT-2),respectively, with a high recovery of 103% on average. The method described, thus, proved suitable for analyzing metallothionein isoform concentrations even in untreated animals. The ratio of MT-1 to MT-2 was found to be 1:1 on average. MT decomposition during storage was very high in whole livers, but could be reduced by about 80% when extracted liver samples were used. (C) 2000 Elsevier Science B.V. All rights reserved.
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