期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 66, 期 8, 页码 3624-3627出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.66.8.3624-3627.2000
关键词
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Fermentation strategies for the production of poly(3-hydroxybutyrate) (PHB) from whey by recombinant Escherichia coli strain CGSC 4401 harboring the Alcaligenes latus polyhydroxyalkanoate (PHA) biosynthesis genes were developed. The pa-stat fed-batch cultures of E. coli CGSC 4401 harboring pJC4, a stable plasmid containing the ii, latus PHA biosynthesis genes, were carried out with a concentrated whey solution containing 280 g of lactose equivalent per liter. Final cell and PHB concentrations of 119.5 and 96.2 g/liter, respectively, were obtained in 37.5 h, which resulted in PHB productivity; of 2.57 g/liter/h.
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