期刊
LETTERS IN APPLIED MICROBIOLOGY
卷 31, 期 2, 页码 118-122出版社
BLACKWELL SCIENCE LTD
DOI: 10.1046/j.1365-2672.2000.00780.x
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The activity of uncultured acidobacteria was monitored in Dutch grassland soils by quantifying their ribosomes. These bacteria were detectable by five different 16S rRNA RT-PCR products in temperature gradient gel electrophoresis fingerprints. The ribosomes in surface soil samples were quantified with multiple competitive RT-PCR along a 1.5-km transect through the grassland. in total, the five members of the acidobacteria were estimated to contribute 4 x 10(10) to 1 x 10(11) ribosomes g soil(-1), representing 7-14% of all bacterial ribosomes. These results indicate that ribosomes from acidobacteria an continuously present and abundant in soil and might contribute significantly to microbial activity in soil.
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