Spectrophotometric and chromatographic determination of sulfanilamides in biological fluids and pharmaceuticals

The conditions for the determination of norsulfazol (I), sulfadimezine (II), ethazole (III), sulfamethoxypyridazine (IV), sulfadimethoxine (V), sodium sulfacyl (VI), sulfalene (VII), sulgin (VIII), and bucarban (IX) as their derivatives of 4,6-dinitrobenzofuroxan using spectrophotometry and thin-layer chromatography were selected. The best results were obtained at 490-500 nm using polar solvents and their aqueous mixtures of pH 6.68 as analytical media. The analytical range for sulfanilamides was 0.5-6.0 mu g/mL. The limits of detection (mu g/mL) were 0.24 for I, 0.28 for II, 0.43 for III, 0.18 for IV, 0.25 for V, 0.54 for VI, 0.54 for VII, 0.17 for VII, and 0.31 for IX. Sulfanilamides were determined in pharmaceuticals (tablets, ointments, and drops) and biological fluids (protein hydrolyzates, urine, blood serum, and whole blood). A simple rapid method for the determination of the phenotype of biotransformation by the type of acetylation was developed based on the studies of the kinetics of the excretion of II from the human body with urine.