期刊
EXPERIMENTAL PARASITOLOGY
卷 95, 期 4, 页码 231-239出版社
ACADEMIC PRESS INC
DOI: 10.1006/expr.2000.4539
关键词
Schistosoma mansoni; trematode; carbohydrates; glycoproteins; glycocalyx; complement system; MBL pathway
类别
Schistosoma mansoni is a blood-dwelling trematode which can persist for several years in the vessels of the human host. The schistosomal surface has been extensively characterized by lectin binding studies, revealing the carbohydrate composition of the worm's tegument. Using fluorescent and scanning electron microscopy we demonstrate that the surface carbohydrates of cercariae and adult worms are the binding ligands for mannan-binding lectin (MBL), a serum protein that is part of the innate immune system. An in vitro complement activation assay with Clq-deficient complement suggests that MEL, in association with the serine proteases MASP-1 and MASP-2, is capable of fixing complement components on the schistosomal tegument and activating the complement cascade via the MBL pathway. MBL is constitutively expressed by hepatocytes and present in the blood at a stable level. Since it is also a weak acute-phase protein and therefore upregulated in an acute-phase response we investigated the serum MBL levels in patients infected with Schistosoma sp. and in healthy control persons. An enzyme-linked immunosorbent assay indicated no differences between the two groups. Although our results suggest an involvement of MBL activated complement in vitro, its role in vivo remains to be clarified. (C) 2000 Academic Press.
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