期刊
NEURON
卷 27, 期 2, 页码 289-299出版社
CELL PRESS
DOI: 10.1016/S0896-6273(00)00037-4
关键词
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资金
- NIGMS NIH HHS [R01 GM059642] Funding Source: Medline
- NINDS NIH HHS [F32 NS009565, NS33187] Funding Source: Medline
Recent studies describe a network of signaling proteins centered around G(o)alpha and G(q)alpha that regulates neurotransmitter secretion in C. elegans by controlling the production and consumption of diacylglycerol (DAG). We sought other components of the G(o)alpha-G(o)alpha signaling network by screening for aldicarb-resistant mutants with phenotypes similar to egl-30 (G(o)alpha) mutants. In so doing, we identified ric-8, which encodes a novel protein named RIC-8 (synembryn). Through cDNA analysis, we show that RIC-8 is conserved in vertebrates. Through immunostaining, we show that RIC-8 is concentrated in the cytoplasm of neurons. Exogenous application of phorbol esters or loss of DGK-1 (diacylglycerol kinase) rescues ric-8 mutant phenotypes. A genetic analysis suggests that RIC-8 functions upstream of, or in conjunction with, EGL-30 (G(o)alpha).
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