期刊
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
卷 25, 期 2, 页码 100-103出版社
NATURE AMERICA INC
DOI: 10.1038/sj.jim.7000039
关键词
Candida magnoliae; erythritol; fed-batch culture
A two-stage fed-batch process was designed to enhance erythritol productivity by the mutant strain of Candida magnoliae, The first stage (or growth stage) was performed in the fed-batch mode where the growth medium was fed when the pH of the culture broth dropped below 4.5, The second stage (or production stage) was started with addition of glucose powder into the culture broth when the cell mass reached about 75 g dry cell weight l(-1), When the initial glucose concentration was adjusted to 400 g l(-1) in the production stage, 2.8 g l(-1) h(-1) of overall erythritol productivity and 41% of erythritol conversion yield were achieved, which represented a fivefold increase in erythritol productivity compared with the simple batch fermentation process. A high glucose concentration in the production phase resulted in formation of organic acids including citrate and butyrate, An increase in dissolved oxygen level caused formation of gluconic acid instead of citric acid.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据