期刊
JOURNAL OF IMMUNOLOGY
卷 165, 期 3, 页码 1479-1485出版社
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.165.3.1479
关键词
-
类别
资金
- NHLBI NIH HHS [HL51630] Funding Source: Medline
- NIAID NIH HHS [N01AI25147] Funding Source: Medline
Protective immunity against Mycobacterium tuberculosis requires CD4(+) lymphocyte-mediated immune responses and IFN-gamma activity. As the primary portal of entry of M, tuberculosis is the lung, pulmonary immune responses against multiple M, tuberculosis Ags were compared between both M, tuberculosis-exposed tuberculin skin test-positive healthy household contacts (HHC) of patients with active sputum smear and culture-positive tuberculosis and tuberculin skin test-positive healthy control individuals from the community (CC), Frequencies of M, tuberculosis Ag-specific IFN-gamma-producing cells, IFN-gamma concentrations in culture supernatants, and DNA synthesis in bronchoalveolar cells (BAC) and PBMC were studied in HHC (n = 10) and CC (n = 15), Using enzyme-linked immunospot assay we found higher frequencies of IFN-gamma-producing cells with specificity to M, tuberculosis-secreted Ag 85 (Ag 85) in BAC from HHC than in BAC from CC (p < 0.022) and relative to autologous PBMC, indicating compartmentalization of Ag 85-specific cells to the lungs. Further, IFN-gamma-producing cells with specificity to components A and B of Ag 85 were specifically compartmentalized to the lungs in HHC (p, < 0,05). IFN-gamma concentrations in culture supernatants of BAC and Ag-specific DNA synthesis were low and comparable in the two subject groups. Increased immune responses to Ag 85 at the site of repeated exposure to M, tuberculosis (the lung) may represent an important component of protective immunity against M, tuberculosis. Correlates of protective immunity against M, tuberculosis are required for assessment of the efficiency of antituberculous vaccines.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据