期刊
BIOLOGICAL PROCEDURES ONLINE
卷 11, 期 1, 页码 253-262出版社
BMC
DOI: 10.1007/s12575-009-9005-0
关键词
Electroporation; Gene delivery; Whole-body imaging; Katushka; Skeletal muscle
资金
- Danish National Advanced Technology Foundation (Hojteknologifonden)
- Danish National Research Foundation [02-512-55]
DNA electrotransfer to muscle tissue yields long-term, high levels of gene expression; showing great promise for future gene therapy. We want to characterize the novel far-red fluorescent protein Katushka as a marker for gene expression using time domain fluorescence in vivo imaging. Highly efficient transgenic expression was observed after DNA electrotransfer with 100-fold increase in fluorescent intensity. The fluorescent signal peaked 1 week after transfection and returned to background level within 4 weeks. Katushka expression was not as stable as GFP expression, which was detectable for 8 weeks. Depth and 3D analysis proved that the expression was located in the target muscle. In vivo bio-imaging using the novel Katushka fluorescent protein enables excellent evaluation of the transfection efficacy, and spatial distribution, but lacks long-term stability.
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