期刊
EMBO JOURNAL
卷 19, 期 15, 页码 3918-3931出版社
OXFORD UNIV PRESS
DOI: 10.1093/emboj/19.15.3918
关键词
nuclear envelope; nuclear lamina; Nup153; Xenopus
In cell-free extracts of Xenopus eggs that support the assembly of replication-competent nuclei, we found that lamin B-3 specifically associates with four polypeptides (termed SLAPs, soluble lamin associated proteins), Here, one SLAP is identified as the nuclear pore complex protein Nup153, one member of the F/GXFG motif-containing nucleoporins. In vitro translated Nup153 and lamin B-3 co-immunoprecipitate, and lamin B-3 interacts specifically with the C-terminal domain of Nup153, During nuclear envelope assembly, other F/GXFG-containing nucleoporins are incorporated into the nuclear envelope preceding lamina assembly. Incorporation of Nup153 occurs at the same time as lamina assembly, When lamina assembly is prevented using the dominant-negative mutant XlaminB Delta 2+, Nup153 does not appear at the nuclear envelope, while other F/GXFG-containing nucleoporins and Nup93 are recruited normally. When the lamina of pre-assembled nuclei is disrupted using the same dominant-negative mutant, the distribution of other nucleoporins is unaffected, However, Nup153 recruitment at the nuclear envelope is lost, Our results indicate that both the recruitment and maintenance of Nup153 at the pore are dependent upon the integrity of the lamina.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据