期刊
ORAL MICROBIOLOGY AND IMMUNOLOGY
卷 15, 期 4, 页码 226-231出版社
MUNKSGAARD INT PUBL LTD
DOI: 10.1034/j.1399-302x.2000.150403.x
关键词
LL-37; cathelicidin; hCAP-18; Actinobacillus actinomycetemcomitans; Ccapnocytophaga spp.
资金
- NIAID NIH HHS [AI43934, AI22839] Funding Source: Medline
- NIDCR NIH HHS [DE11843, DE08161] Funding Source: Medline
The bactericidal activity of synthetic LL-37, a cathelicidin, was assessed against Actinobacillus actinomyoetemcomitans (three strains) and Capnocytophaga spp. (three strains). All strains were sensitive to LL-37, and exhibited 99% effective dose of 7.5-to-11.6 mu g/ml. An amidated form of LL-37, pentamide-37, killed with about the same efficacy as LL-37. Partial inhibition of killing was noted at physiologic concentrations of NaCl, and complete inhibition was observed at 400 mM NaCl. At approximately the 99% effective dose - i.e., 10 mu g/ml - LL-37 also lost activity against A. actinomycetemcomitans in the presence of native or heat-inactivated 10-15% normal human AB serum. Pentamide-37 was less sensitive to serum inhibition than LL-37. In conclusion, certain oral, gram-negative bacteria are sensitive to the bactericidal activity of LL-37 at low concentrations of serum and salt, a condition likely to be found within the membrane-delimited phagolysosome. Modified forms of LL-37, such as pentamide-37, may be more suitable for future therapeutic application in the presence of serum.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据