期刊
MECHANISMS OF DEVELOPMENT
卷 96, 期 1, 页码 79-89出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0925-4773(00)00377-4
关键词
Hoxd4; retinoic acid; regulatory element; gene expression; gene regulation; transgenic mouse; pattern formation
We have identified a retinoic acid response element (RARE) within a neural enhancer located 3' to the Hoxd4 gene. This RARE is required for the initiation and maintenance of Hoxd4 transgene expression in neurectoderm, and for full anteriorized expression upon retinoic acid (RA) treatment. Mutations within the sequence TTTTCTG, located 2 bp downstream of the RARE, posteriorized transgene activity. However, the onset of transgene expression and its response to RA were indistinguishable from wild type. While the TTTTCTG motif resembles a CDX binding site, human CDX1 protein did not interact with this element in vitro. Three additional regions were also shown to control transgene expression in neurectoderm, establishing that multiple elements constitute the Hoxd4 neural enhancer. (C) 2000 Elsevier Science ireland Ltd. All rights reserved.
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