期刊
ANALYTICA CHIMICA ACTA
卷 888, 期 -, 页码 182-190出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2015.06.058
关键词
Mixed-mode chromatography; Two-dimensional liquid chromatography on the single column; Reversed phase liquid chromatography; Hydrophilic interaction chromatography
资金
- National Natural Science Foundation of China [21177099]
- Program for New Century Excellent Talents in University [NCET-12-0213]
A mixed-mode chromatographic packing material, C18 and diol groups modified silica (C18-Diol), was prepared with controllable hydrophobicity and hydrophilicity. It demonstrated excellent aqueous compatibility and stability in aqueous mobile phase; compared to the traditional C18 column, improved peak shape of basic analytes was also obtained. Additionally, it exhibited both reversed-phase liquid chromatographic (RPLC) and hydrophilic interaction chromatographic (HILIC) performance; the analyte separation scope was thus enlarged, demonstrated by simultaneous separation of twenty acids, bases and neutrals. More interestingly, a novel on-line two-dimensional liquid chromatography on the single column (2D-LC-1C) was established by modifying the high performance liquid chromatographic instrument only with the addition of an extra six-port two-position valve. The early co-eluted components of the extract of Lonicera japonica on the 1st-dimension (RPLC) were collected for the online re-injection to the 2nd-dimension (HILIC) by conveniently varying the mobile phase components. Six more peaks were obtained. The established system was simple, easy operation and low cost, which had advantages in analyzing complicated samples. (C) 2015 Elsevier B.V. All rights reserved.
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