期刊
BRAIN RESEARCH
卷 873, 期 1, 页码 135-139出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0006-8993(00)02476-8
关键词
calcium-binding protein; nucleus magnocellularis; in situ hybridization; N06 antibody; intracellular calcium concentration; cochlea removal
资金
- NIDCD NIH HHS [DC02633] Funding Source: Medline
The expression of the calcium-binding protein calretinin (CR) in the chick cochlear nucleus, nucleus magnocellularis (NM), was examined after unilateral cochlea removal in hatchlings and 3-week-old birds. Following survival times of 3, 6 or 12 h or 1, 3 or 7 days, CR mRNA was localized by in situ hybridization using a radiolabeled chick CR probe. Levels of CR protein were assessed by CR-immunoreactivity (CR-I) using a calcium-independent CR antiserum and immunohistochemistry. At survival times of 1 day or less, cochlea removal did not change relative levels of CR mRNA or CR-I in NM between the deafferented and the unoperated sides of the brain. Decreases in CR expression on the cochlea removal side were observed, however, at times that coincide with deafferentation-induced cell loss (3 and 7 days). Additionally, bilateral cochlea removal had no apparent effect on CR-I in NM neurons when compared to those in normal animals. Thus, CR mRNA and protein appear to be constitutively expressed in NM neurons and are not affected by sensory deprivation. (C) 2000 Elsevier Science B.V. All rights reserved.
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