期刊
ANALYTICA CHIMICA ACTA
卷 418, 期 2, 页码 119-128出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0003-2670(00)00952-1
关键词
bacteria; pathogens; biomarker; fatty acid; FAME; pyrolysis; ion trap MS; CI; tert-butyl bromide
Fatty acid methyl esters (FAMEs) from 20 bacterial samples, formed through in situ thermal hydrolysis and methylation (THM) of lipids from whole bacterial cells using tetramethylammonium hydroxide, have been investigated by t-butyl bromide chemical ionization (CI) ion trap mass spectrometry (MS). The mass spectra, in the early portion of the total ion profile, contained as major peaks the protonated saturated and unsaturated FAME molecular ions, along with the fragment ions, [MH-32](+) and [MH-50](+), from the monoenoic FAME isomers. The data set was tested with several multivariate statistical approaches. Gram-type classification was made based on the ratio of saturated fatty acids (mainly C15:0) to monoenoic fatty acids (mainly C18:1, C16:1, and cyclopropyl fatty acids, C17:0 and C19:0). A multivariate rule building expert system (MuRES) was used to classify targeted bacteria comprising five different genera. Cross validation of the data set showed no false classifications of the targeted organisms. Misclassifications of untargeted bacteria as target organisms were low (1.3%). The mass spectral analysis time was less than 5 min per sample. (C) 2000 Elsevier Science B.V. All rights reserved.
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