Facile, Fmoc-compatible solid-phase synthesis of peptide C-terminal thioesters

[GRAPHICS] A short route to peptide C terminal thioesters was developed that does not require the use of special linkers or resins and is compatible with standard Fmoc chemistry, Following conventional solid phase peptide synthesis, an excess of Me2AlCl and EtSH in dichloromethane cleaves peptides from Wang or Pam resins to give the corresponding thioesters directly in good yield and purity.